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HelpTest Code PBNP1 NT-Pro B-Type Natriuretic Peptide, Serum
Necessary Information
Patient's age and sex are required.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.
Testing Algorithm
For more information see Amyloidosis: Laboratory Approach to Diagnosis
Method Name
Electrochemiluminescence Immunoassay
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 365 days |
| Refrigerated | 7 days |
Reject Due To
| Gross hemolysis | Reject |
Clinical Information
B-type natriuretic peptide (formerly brain natriuretic peptide: BNP) is a small, ringed peptide secreted by the heart to regulate blood pressure and fluid balance.(1) This peptide is stored in, and secreted predominantly from, membrane granules in the heart ventricles in a pro form (proBNP). Once released from the heart in response to ventricle volume expansion or pressure overload, the N-terminal (NT) piece of 76 amino acids (NT-proBNP) is rapidly cleaved by the enzymes corin and furin to release the active 32-amino acid peptide (BNP).(2)
Both BNP and NT-proBNP are markers of atrial and ventricular distension due to increased intracardiac pressure. The New York Heart Association (NYHA) developed a 4-stage functional classification system for congestive heart failure (CHF) based on the severity of the symptoms. Studies have demonstrated that the measured concentrations of circulating BNP and NT-proBNP increase with the severity of CHF based on the NYHA classification.
Reference Values
Males
0-2 day: 321-11,987 pg/mL
3-11 day: 263-5918 pg/mL
12 day-1 month: Not applicable
2 month-1 year: 37-646 pg/mL
2 years: 39-413 pg/mL
3 years-6 years: 23-289 pg/mL
7 years-14 years: ≤157 pg/mL
15 years-18 years: ≤158 pg/mL
19-39 years: <79 pg/mL
40-44 years: ≤72 pg/mL
45-54 years: ≤87 pg/mL
55-64 years: ≤88 pg/mL
≥65 years: ≤540 pg/mL
Females
0-2 day: 321-11,987 pg/mL
3-11 day: 263-5918 pg/mL
12 day-1 month: Not applicable
2 month-1 year: 37-646 pg/mL
2 years: 39-413 pg/mL
3 years-6 years: 23-289 pg/mL
7 years-14 years: < or=157 pg/mL
15 years-18 years: ≤158 pg/mL
19-39 years: <160 pg/mL
40-44 years: ≤162 pg/mL
45-54 years: ≤141 pg/mL
55-64 years: ≤226 pg/mL
≥65 years: ≤540 pg/mL
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 2 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
83880
Forms
If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen.