-1739993209.png){kind=logo}
Home
HelpTest Code MPHNU Methylphenidate and Metabolite, Random, Urine
Additional Testing Requirements
If urine creatinine is required or adulteration of the sample is suspected, also order ADULT / Adulterants Survey, Random, Urine. For more information, see ADULT / Adulterants Survey, Random, Urine.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube: Plastic urine container
Submission Container/Tube: Plastic, 5-mL tube
Specimen Volume: 5 mL
Collection Instructions:
1. Collect a random urine specimen.
2. No preservative.
Additional Information:
1. No specimen substitutions.
2. STAT requests are not accepted for this test.
3. Submitting less than 1 mL will compromise the ability to perform all necessary testing.
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Specimen Type
UrineSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 10 days |
| Frozen | 28 days |
Reject Due To
| Gross hemolysis | OK |
| Gross icterus | OK |
Clinical Information
Methylphenidate (MPH) is utilized for the treatment of attention-deficit hyperactivity disorder and narcolepsy. MPH has two chiral centers and is marketed as a racemic mixture and as the active d-enantiomer of racemic MPH. Although the exact mechanism of its action has not been fully defined, it blocks the reuptake of norepinephrine and dopamine into the presynaptic neuron thus increasing the concentrations of these monoamines in the extraneural space.
Reference Values
Negative (Positive results are reported with a quantitative result.)
Cutoff concentrations by liquid chromatography tandem mass spectrometry:
Methylphenidate: 10 ng/mL
Ritalinic Acid: 50 ng/mL
Day(s) Performed
Monday, Wednesday, Friday
Report Available
2 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
G0480
80360 (if appropriate for select payers)
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.