Test Code MMGEN Mycoplasma genitalium, Transcription-Mediated Amplification, Post-Prostatic Massage Fluid/Urine or Peritoneal Fluids
Necessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Post-prostatic massage fluid/urine (VBIII)
Supplies: Aptima Urine Transport Tube (T582)
Container/Tube: Aptima Urine Specimen Transport Tube
Specimen Volume: 15 to 20 mL
Collection Instructions:
1. Patient should not have urinated for at least 1 hour prior to specimen collection.
2. Patient should void a small amount of urine prior to prostatic massage. Pre-massage urine can be discarded.
3. Patient should cease voiding and a prostatic massage should be performed by urologist or other healthcare professional.
4. Collect post-massage urine into a sterile, plastic, preservative-free container.
5. Transfer 2 mL of urine into the Aptima Urine Specimen Transport Tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the urine transport tube.
6. Place the labels on the transport tube so the black fill lines are still visible for volume confirmation at Mayo Clinic Laboratories.
7. Transport and store post-prostatic massage fluid/urine (VBIII) specimen transport container at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. Specimen cannot be sent frozen.
Specimen Type: Peritoneal fluid (pelvic wash, cul-de-sac fluid)
Supplies: Aptima Specimen Transfer Kit (T652)
Container/Tube: Aptima Specimen Transfer Tube
Specimen Volume: 1 mL
Collection Instructions:
1. Collect specimen by paracentesis using needle and syringe using routine procedures. Refer to the references below.
2. Thoroughly vortex specimen upon receipt to the laboratory.
3. Transfer 1 mL of peritoneal fluid into the Aptima Specimen Transfer kit using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug) within 24 hours of collection.
4. Recap the specimen transfer tube tightly.
5. Transport and store specimen container at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. Specimen cannot be sent frozen.
Method Name
Transcription-Mediated Amplification
Specimen Type
VariesSpecimen Minimum Volume
Peritoneal fluid: 1 mL; Post-prostatic massage fluid/urine (VBIII): See Specimen Required
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Varies | APTIMA VIAL |
Reject Due To
Overfilled or underfilled urine transport tubes Specimen collected into a SurePath Prep device or ThinPrep vial Midstream/clean catch urine specimen Transport tubes contain swabs Multiple sources on single tube |
Reject |
Clinical Information
Mycoplasma genitalium, an under-recognized sexually transmitted infection (STI), causes acute and chronic non-gonococcal urethritis, cervicitis, and pelvic inflammatory disease. Due to its growing prevalence, M genitalium was cited as an emerging public health threat by the Centers for Disease Control and Prevention (CDC) in 2015. In high-risk populations, prevalence has been reported as high as 9% to 24% in male patients and 11% to 16% in female patients. M genitalium is commonly misdiagnosed as other STIs (eg, Chlamydia trachomatis or gonorrhea), which can lead to improper treatment of the underlying cause and an increase in duration of infection. In 2021, the CDC updated their STI guidelines to recommend that men with recurrent non-gonococcal urethritis and women with recurrent cervicitis or pelvic inflammatory disease should be tested for M genitalium.
Reference Values
Negative
Day(s) Performed
Monday through Sunday
Report Available
1 to 4 daysPerforming Laboratory

CPT Code Information
87563
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.