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Test Code BARBX Barbiturates Confirmation, Chain of Custody, Random, Urine

Additional Tests

Test ID Reporting Name Available Separately Always Performed
COCH Chain of Custody Processing No Yes
ADLTX Adulterants Survey, CoC, U Yes Yes

Testing Algorithm

Adulterants testing will be performed on all chain-of-custody urine samples as per regulatory requirements.

Specimen Type

Urine


Specimen Required


Supplies: Chain of Custody Kit (T282)

Container/Tube: Chain of custody kit (T282) containing the specimen containers, seals, and documentation required

Specimen Volume: 10 mL

Collection Instructions: Collect specimen in the container provided, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.

Additional Information: Submitting less than 10 mL will compromise the ability to perform all necessary testing.


Specimen Minimum Volume

5 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  72 hours

Reject Due To

Gross hemolysis OK
Gross icterus OK

Clinical Information

Barbiturates represent a class of drugs that were originally introduced as sleep inducers. Butalbital is also used to control severe headaches. Mephobarbital and phenobarbital are frequently used to control major motor (grand mal) seizures. These drugs are commonly abused as "downers" to induce sleep after an amphetamine- or cocaine-induced "high."

 

Chain of custody is a record of the disposition of a specimen to document the personnel who collected, handled, and performed the analysis. When a specimen is submitted in this manner, analysis will be performed in such a way that it will withstand regular court scrutiny.

Reference Values

Negative

Positive results are reported with a quantitative result.

 

Cutoff concentrations:

Immunoassay screen: 200 ng/mL

 

Gas chromatography mass spectrometry:

Butalbital: 100 ng/mL

Amobarbital: 100 ng/mL

Pentobarbital: 100 ng/mL

Secobarbital: 100 ng/mL

Phenobarbital: 100 ng/mL

Day(s) Performed

Monday, Wednesday, Friday

Report Available

3 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80345

G0480 (if appropriate)

Method Name

Immunoassay/Gas Chromatography Mass Spectrometry (GC-MS) Confirmation with Quantitation

Forms

1. Chain of Custody Request is included in the Chain of Custody Kit (T282).

2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.