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Test Code ALCX Ethanol, Chain of Custody, Blood

Additional Tests

Test ID Reporting Name Available Separately Always Performed
COCH Chain of Custody Processing No Yes

Specimen Type

Whole Blood NaFl-KOx


Specimen Required


Supplies: Chain-of-Custody Kit (T282)

Container/Tube: Chain-of-Custody Kit (T282) containing the specimen container seals and documentation required.

Preferred: Gray top (potassium oxalate/sodium fluoride)

Acceptable: Lavender (EDTA) or green top (sodium heparin)

Specimen Volume: 2 mL

Collection Instructions:

1. Do not use alcohol to clean arm. Use an alternative, such as Betadine, to cleanse arm before collecting any specimen for volatile testing.

2. Specimen must be sent in original tube. Collect specimen, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.


Specimen Minimum Volume

0.5 mL or amount to fill 1 tube

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood NaFl-KOx Refrigerated (preferred) 14 days
  Frozen  28 days
  Ambient  24 hours

Reject Due To

Gross lipemia Reject
Gross icterus OK

Clinical Information

Ethanol is one of the most widely abused legal substances in the United States. It is the active agent in beer, wine, vodka, whiskey, rum, and other liquors.

 

Ethanol acts on cerebral functions as a depressant similar to general anesthetics. This depression causes most of the typical symptoms such as impaired thought, clouded judgment, and changed behavior. As the level of alcohol increases, the degree of impairment becomes progressively increased.

 

In most jurisdictions in the United States, the level of prima facie evidence of being under the influence of alcohol for purposes of driving a motor vehicle is 80 mg/dL (0.08%) in whole blood.

 

Chain of custody is a record of the disposition of a specimen to document the personnel who collected it, handled it, and performed the analysis. When a specimen is submitted in this manner, analysis will be performed in such a way that it will withstand regular court scrutiny.

Reference Values

Not detected (Positive results are quantified.)

Limit of detection: 10 mg/dL (0.01 g/dL)

Legal limit of intoxication is 80 mg/dL (0.08 g/dL).

Toxic concentration is dependent upon individual usage history.

Potentially lethal concentration: ≥400 mg/dL (0.4 g/dL)

Day(s) Performed

Monday through Saturday

Report Available

1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80320

G0480 (if appropriate)

Method Name

Headspace Gas Chromatography with Flame Ionization Detector (HSGC-FID)

Forms

1. Chain of Custody Request is included in the Chain-of-Custody Kit (T282).

2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.