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HelpTest Code ABIDR Antibody Identification, Blood and Serum
Shipping Instructions
Specimen must arrive within 72 hours of collection.
Specimen Required
Both blood and serum are required.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Specimen Type: Plasma/Blood
Collection Container/Tube: 6-mL Pink-top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume:
3 mL Plasma
3 mL Red blood cells (RBCs)
Collection Instructions:
1. Centrifuge and aliquot plasma into a plastic vial.
2. Label specimen as EDTA plasma or EDTA RBCs as appropriate.
3. Send both tubes.
Specimen Type: Serum/Blood
Collection Container/Tube: 10-mL Red top
Submission Container/Tube: Plastic vial
Specimen Volume:
5 mL Serum
5 mL RBCs
Collection Instructions:
1. Centrifuge and aliquot serum into a plastic vial.
2. Label specimen as serum or clotted RBCs as appropriate.
3. Send both tubes.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| STTX26 | Antibody Panel | No, (Bill Only) | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| DCTR | Direct Antiglobulin Test (Poly) | Yes | No |
| SPAGR | Special Red Cell Ag Typing | Yes | No |
| ABTIR | Antibody Titer, RBC | Yes | No |
| STTX25 | Antibody Elution | No, (Bill Only) | No |
| STTX31 | Antibody Adsorption | No, (Bill Only) | No |
| STTX32 | Red Cell Antigen Typing | No, (Bill Only) | No |
Testing Algorithm
The following tests may be ordered and performed (at an additional charge) as needed for antibody identification: direct antiglobulin testing (polyspecific), including its reflex tests and special red blood cell antigen typing.
If certain antibodies are detected and the patient is known to be pregnant, an antibody titration will be performed at an additional charge.
Method Name
Hemagglutination
Specimen Type
VariesSpecimen Minimum Volume
Blood: 6 mL in EDTA
Pediatric: 2 mL Serum
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | 4 days |
| Refrigerated | 4 days |
Reject Due To
| Gross hemolysis | OK |
Clinical Information
After exposure to foreign red blood cells (RBCs) via transfusion or pregnancy, some people form antibodies that are capable of the destruction of transfused RBCs or of fetal RBCs in utero. It is important to identify the antibody specificity to assess the antibody's capability of causing clinical harm and, if necessary, to avoid the antigen on transfused RBCs.
Autoantibodies react against the patient's own RBCs as well as the majority of cells tested. Autoantibodies can be clinically benign or can hemolyze the patient's own RBCs, such as in cold agglutinin disease or autoimmune hemolytic anemia.
Reference Values
Negative
If positive, antibodies will be identified and corresponding special red cell antigen typing on patient’s red blood cells will be performed.
Day(s) Performed
Monday through Friday, Sunday
Report Available
1 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86870
86860-(if appropriate)
86886-(if appropriate)
86880 x 3 (if appropriate)
86905-(if appropriate)
86978-(if appropriate)